Description: The Anti-6xHis recombinant mouse monoclonal antibody (Clone N144/14R) is designed for specific binding to the 6xHis tag. This tag is commonly used in protein purification and detection due to its high affinity for nickel and cobalt ions.
Table of Antibody Characteristics:
| Attribute | Details |
|---|---|
| Antibody Clone | N144/14R |
| Applications | Immunocytochemistry, Western Blot |
| Clonality | Monoclonal |
| Host | Mus musculus (Mouse) |
| Immunogen | 6xHis fusion protein (Amino acids 720–804 of mouse ProtocadherinGammaA3) |
| Isotype | IgG2a |
| Purification | Protein A or G affinity columns, buffer exchange |
Usage and Storage:
- Concentration: 0.9–1.1 mg/mL
- Storage: Store at -20 °C in single-use aliquots. Avoid multiple freeze-thaw cycles.
- Shipment: Shipped on blue ice or at room temperature.
Target Antigen:
- Protein 6x HIS (Hexahistidine)
- Recognizes various 6xHis-tagged fusion proteins via ELISA, immunofluorescence, and immunoblotting.
Assay Compatibility:
- Effective in Immunocytochemistry and Western Blot applications.
- Tested on human samples and other species with successful results.
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