Hi T7 RNA polymerase

T7 RNA polymerase is a bacteriophage-derived enzyme with high specificity for its promoter sequence. It catalyzes transcription from DNA to RNA, making it a valuable tool in molecular biology. Particularly, Hi T7 polymerase refers to a modified or engineered version, often exhibiting enhanced performance, efficiency, or specialized features for specific research or applications, optimizing RNA synthesis in molecular studies.

Reaction volume: 20uL; Production rate: 80-100ug RNA. 

10xHi T7 TransBuffer                              2 μl

rNTP Mixture (25 mM each)                  3.2 μl

Linearized template DNA                       0.5-1 μg

Hi T7 RNA Polymerase (100 U/μl)      0.5-1 μl

DEPC-treated Water up to                     20μl