![Anti-GFP [N86/38.1R]](https://rna-vac.com/wp-content/uploads/2023/12/antiGFP-Immunocytochemistry2.jpg "antiGFP-Immunocytochemistry2")
Description: The Anti-GFP antibody is a recombinant mouse monoclonal antibody targeting the Green Fluorescent Protein (GFP) from Aequorea victoria. It cross-reacts with Cyan and Yellow fluorescent proteins (CFP and YFP, respectively).
This antibody also available on other mouse or human IgG isotype upon request.
Table of Specifications:
| Feature | Specification |
|---|---|
| Antibody Clone | N86/38.1R |
| Applications | Immunohistochemistry, Immunocytochemistry, Western Blot |
| Clonality | Monoclonal |
| Host | M. musculus (mouse) |
| Immunogen | Amino acids 1–238 (full-length) of jellyfish green fluorescent protein |
| Isotype | IgG2a |
| Light Chain | kappa |
| Purification | Purified from cell culture supernatant using Protein A or Protein G affinity columns followed by buffer exchange with centrifugal columns |
Application Notes for Anti-GFP Monoclonal Antibody (Clone N86/38.1R):
- Immunofluorescence: Successfully used in mouse brain tissues, showing binding to both yellow and green fluorescent proteins (Kumar et al, 2015; PMID:25337655).
- Immunoprecipitation & Western Blotting: Effective in studies with various Drosophila fly strains, including Elav-GAL4, 50Y-GAL4, UAS-eGFP, UAS-GFP, and RpL10A (Thomas et al, 2012; PMID:22792260).
- Immunohistochemistry: Proven utility in zebrafish for GFP visualization (Mackenzie and Raible, 2012; PMID:23094043).
- Additional Immunoprecipitation: Applied on sciatic nerves of rats infected with a GFP-containing adenovirus (Chang et al, 2014; PMID:25362471).
These studies demonstrate the antibody’s versatility and efficacy across various species and applications.
Antigen Description: The antigen is the full-length jellyfish GFP, comprising amino acids 1–238. The specific amino acid sequence is detailed as follows: MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
Reviews
There are no reviews yet.